Detection of vanA and vanB2-containing enterococci from food samples in Spain, including Enterococcus faecium strains of CC17 and the new singleton ST425

  1. López, M. 1
  2. Sáenz, Y. 2
  3. Rojo-Bezares, B. 2
  4. Martínez, S. 1
  5. del Campo, R. 3
  6. Ruiz-Larrea, F. 1
  7. Zarazaga, M. 1
  8. Torres, C. 1
  1. 1 Universidad de La Rioja

    Universidad de La Rioja

    Logroño, España


  2. 2 Centro de Investigación Biomédica de La Rioja

    Centro de Investigación Biomédica de La Rioja

    Logroño, España


  3. 3 Hospital Ramón y Cajal

    Hospital Ramón y Cajal

    Madrid, España


International Journal of Food Microbiology

ISSN: 0168-1605

Any de publicació: 2009

Volum: 133

Número: 1-2

Pàgines: 172-178

Tipus: Article

DOI: 10.1016/J.IJFOODMICRO.2009.05.020 SCOPUS: 2-s2.0-67649083992 WoS: WOS:000268219600023 GOOGLE SCHOLAR

Altres publicacions en: International Journal of Food Microbiology


Cites rebudes

  • Cites en Scopus: 65 (09-09-2023)
  • Citas en Web of Science: 57 (07-09-2023)
  • Cites en Dimensions: 58 (30-03-2023)

JCR (Journal Impact Factor)

  • Any 2009
  • Factor d'impacte de la revista: 3.011
  • Factor d'impacte sense autocites: 2.619
  • Article influence score: 0.829
  • Quartil major: Q1
  • Àrea: FOOD SCIENCE & TECHNOLOGY Quartil: Q1 Posició en l'àrea: 8/118 (Edició: SCIE)
  • Àrea: MICROBIOLOGY Quartil: Q2 Posició en l'àrea: 33/95 (Edició: SCIE)

SCImago Journal Rank

  • Any 2009
  • Impacte SJR de la revista: 1.475
  • Quartil major: Q1
  • Àrea: Food Science Quartil: Q1 Posició en l'àrea: 12/244
  • Àrea: Safety, Risk, Reliability and Quality Quartil: Q1 Posició en l'àrea: 5/293
  • Àrea: Microbiology Quartil: Q1 Posició en l'àrea: 28/130
  • Àrea: Medicine (miscellaneous) Quartil: Q1 Posició en l'àrea: 235/2938


(Dades actualitzats a data de 30-03-2023)
  • Cites totals: 58
  • Cites recents: 3
  • Relative Citation Ratio (RCR): 1.94
  • Field Citation Ratio (FCR): 4.2


Two-hundred-twenty-nine food samples of animal origin were tested to know the prevalence of vancomycin-resistant enterococci (VRE) after a decade of avoparcin ban as animal growth promoter in Spain. VRE with acquired mechanism of resistance were detected in 9 of these 229 samples (3.9%, obtained from chicken, veal and rabbit), and one VRE per food sample was further characterized. The vanA gene was identified in seven isolates (2 E. faecium, 3 E. durans, and 2 E. hirae), and the vanB2 gene in the remaining 2 isolates (identified as E. faecium). The two vanB2 isolates showed a phenotype of multiresistance that included, in addition to vancomycin, also ampicillin, erythromycin, tetracycline, streptomycin, kanamycin, ciprofloxacin, chloramphenicol and trimethoprim-sulfamethoxazole and contained, among others, erm(B), tet(M), ant(6), and aph(3′)-III genes. Most of vanA enterococci showed erythromycin and tetracycline resistance and contained the erm(B) and tet(M) genes. One vanA- and both vanB2-positive E. faecium isolates were classified by MLST analysis into the CC17 clonal complex (ST17 and ST78), and one additional vanA isolate was included in a new sequence type named ST425 (singleton). Co-transference by conjugation of erm(B) and vanA genes was demonstrated in one vanA-positive E. faecium isolate. The inclusion of vanB2 cluster into Tn5382 structure was demonstrated in the two vanB2 isolates, as well as the linkage pbp5-Tn5382, and beta-haemolysis and gelatinase production was identified in one of them. Food sample of animal origin could be a vehicle of transference of VRE of vanA and vanB2 type that could be transferred to humans. © 2009 Elsevier B.V. All rights reserved.