Intestinal colonization by vanA or vanB2-containing enterococcal isolates of healthy animals in Spain

  1. Torres, C. 1
  2. Tenorio, C. 1
  3. Portillo, A. 1
  4. García, Maite A.. 1
  5. Martínez, Carolina. 1
  6. Del Campo, R. 1
  7. Ruiz-Larrea, F. 1
  8. Zarazaga, M. 1
  1. 1 Universidad de La Rioja

    Universidad de La Rioja

    Logroño, España


Microbial Drug Resistance

ISSN: 1076-6294

Year of publication: 2003

Volume: 9 Suppl 1

Issue: (Suppl 1:S)

Pages: 47-52

Type: Article

More publications in: Microbial Drug Resistance


Cited by

  • Scopus Cited by: 68 (08-05-2023)
  • Web of Science Cited by: 59 (12-05-2023)

JCR (Journal Impact Factor)

  • Year 2003
  • Journal Impact Factor: 2.32
  • Journal Impact Factor without self cites: 2.134
  • Article influence score: 0.0
  • Best Quartile: Q2
  • Area: PHARMACOLOGY & PHARMACY Quartile: Q2 Rank in area: 57/185 (Ranking edition: SCIE)
  • Area: MICROBIOLOGY Quartile: Q2 Rank in area: 28/84 (Ranking edition: SCIE)
  • Area: INFECTIOUS DISEASES Quartile: Q2 Rank in area: 11/41 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2003
  • SJR Journal Impact: 1.303
  • Best Quartile: Q1
  • Area: Microbiology Quartile: Q1 Rank in area: 24/109
  • Area: Immunology Quartile: Q1 Rank in area: 38/175
  • Area: Microbiology (medical) Quartile: Q1 Rank in area: 9/100
  • Area: Medicine (miscellaneous) Quartile: Q1 Rank in area: 162/2980
  • Area: Pharmacology Quartile: Q1 Rank in area: 36/304


Fecal samples of healthy animals (66 pigs, 22 pets) recovered during 1998 in La Rioja, Spain, were analyzed for vancomycin-resistant enterococci colonization. Vancomycin resistance mechanisms were analyzed by PCR and sequencing. vanA-containing enterococci were detected in 3 of 66 samples (4.5%) and 5 of 22 samples (22.7%) of the pig and pet samples, respectively. Seven unrelated pulsed-field gel electrophoresis (PFGE) patterns were detected among the 8 vanA isolates (7 Enterococcus faecium, 1 E. faecalis). The tet(M) gene was present in all eight vanA enterococcal isolates, while the erm(B) and aac(6')-Ie-aph(2")-Ia genes were detected in 6 and 3 isolates, respectively. Colonization by vanC-1-containing enterococci (E. gallinarum) was demonstrated in 3% and 4.5% of the pig and pet samples. The aac(6')-Ie-aph(2")-Ia, ant(6)-Ia, aph(3')-IIIa, erm(B) and tet(M) genes were identified in one of the E. gallinarum isolates from a pig fecal sample. One vanB2-containing E. hirae strain was detected in the fecal sample of a healthy pig. In this isolate, the vanB2 gene cluster was integrated into the Tn5382-like element, as demonstrated by specific PCRs and sequencing. The tet(M) and erm(B) genes were also detected in this isolate. This is the first report in which a vanB2-containing enterococci is detected in animals and in E. hirae.