Multilocus sequence Typing Scheme for Enterococucus faecalis Reveals Hospital-Adapted genetic Complexes in Background of High Rates of Recombination

  1. Ruiz-Garbajosa, P. 5
  2. Bonten, M.J.M. 4
  3. Robinson, D.A. 3
  4. Top, J. 4
  5. Nallapareddy, S.R. 1
  6. Torres, C. 2
  7. Coque, T.M. 5
  8. Cantón, R. 5
  9. Baquero, F. 5
  10. Murray, B.E. 1
  11. Del Campo, R. 5
  12. Willems, R.J.L. 4
  1. 1 University of Texas System

    University of Texas System

    Austin, Estados Unidos


  2. 2 Universidad de La Rioja

    Universidad de La Rioja

    Logroño, España


  3. 3 New York Medical College

    New York Medical College

    Valhalla, Estados Unidos


  4. 4 University Medical Center Utrecht

    University Medical Center Utrecht

    Utrecht, Holanda


  5. 5 Hospital Ramón y Cajal

    Hospital Ramón y Cajal

    Madrid, España


Journal of Clinical Microbiology

ISSN: 0095-1137

Año de publicación: 2006

Volumen: 44

Número: 6

Páginas: 2220-2228

Tipo: Artículo

DOI: 10.1128/JCM.02596-05 PMID: 16757624 SCOPUS: 2-s2.0-33745136790 WoS: WOS:000238332900044 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Clinical Microbiology

Archivo institucional: lock_openAcceso abierto editor


Citas recibidas

  • Citas en Scopus: 297 (09-09-2023)
  • Citas en Web of Science: 273 (07-09-2023)
  • Citas en Dimensions: 310 (30-03-2023)

JCR (Journal Impact Factor)

  • Año 2006
  • Factor de impacto de la revista: 3.445
  • Factor de impacto sin autocitas: 2.884
  • Article influence score: 0.0
  • Cuartil mayor: Q1
  • Área: MICROBIOLOGY Cuartil: Q1 Posición en el área: 21/89 (Edicion: SCIE)

SCImago Journal Rank

  • Año 2006
  • Impacto SJR de la revista: 2.212
  • Cuartil mayor: Q1
  • Área: Microbiology (medical) Cuartil: Q1 Posición en el área: 7/102


(Datos actualizados a fecha de 30-03-2023)
  • Citas totales: 310
  • Citas recientes: 45
  • Relative Citation Ratio (RCR): 7.76
  • Field Citation Ratio (FCR): 37.3


A multilocus sequence typing (MLST) scheme based on seven housekeeping genes was used to investigate the epidemiology and population structure of Enterococcus faecalis. MLST of 110 isolates from different sources and geographic locations revealed 55 different sequence types that grouped into four major clonal complexes (CC2, CC9, CC10, and CC21) by use of eBURST. Two of these clonal complexes, CC2 and CC9, are particularly fit in the hospital environment, as CC2 includes the previously described BVE clonal complex identified by an alternative MLST scheme and CC9 includes exclusively isolates from hospitalized patients. Identical alleles were found in genetically diverse isolates with no linkage disequilibrium, while the different MLST loci gave incongruent phylogenetic trees. This demonstrates that recombination is an important mechanism driving genetic variation in E. faecalis and suggests an epidemic population structure for E. faecalis. Our novel MLST scheme provides an excellent tool for investigating local and short-term epidemiology as well as global epidemiology, population structure, and genetic evolution of E. faecalis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.