Protein fingerprinting of Staphylococcus species by capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection

  1. Veledo, M.T. 1
  2. Pelaez-Lorenzo, C. 1
  3. Gonzalez, R. 2
  4. de Frutos, M. 1
  5. Diez-Masa, J.C. 1
  1. 1 Instituto de Química Orgánica General

    Instituto de Química Orgánica General

    Madrid, España


  2. 2 Instituto de Ciencias de la Vid y del Vino

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España


Analytica Chimica Acta

ISSN: 0003-2670

Year of publication: 2010

Volume: 658

Issue: 1

Pages: 81-86

Type: Article

DOI: 10.1016/J.ACA.2009.10.053 PMID: 20082778 SCOPUS: 2-s2.0-71749121611 WoS: WOS:000273847500011 GOOGLE SCHOLAR

More publications in: Analytica Chimica Acta


Cited by

  • Scopus Cited by: 12 (06-06-2023)
  • Web of Science Cited by: 11 (19-05-2023)
  • Dimensions Cited by: 12 (30-03-2023)

JCR (Journal Impact Factor)

  • Year 2010
  • Journal Impact Factor: 4.311
  • Journal Impact Factor without self cites: 3.993
  • Article influence score: 0.897
  • Best Quartile: Q1
  • Area: CHEMISTRY, ANALYTICAL Quartile: Q1 Rank in area: 6/73 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2010
  • SJR Journal Impact: 1.703
  • Best Quartile: Q1
  • Area: Biochemistry Quartile: Q1 Rank in area: 79/396
  • Area: Spectroscopy Quartile: Q1 Rank in area: 8/65
  • Area: Analytical Chemistry Quartile: Q1 Rank in area: 10/103
  • Area: Environmental Chemistry Quartile: Q1 Rank in area: 12/107


(Data updated as of 30-03-2023)
  • Total citations: 12
  • Recent citations: 1
  • Relative Citation Ratio (RCR): 0.39
  • Field Citation Ratio (FCR): 0.9


Capillary electrophoresis (CE) coupled with laser-induced fluorescence detection (LIF) has allowed to obtain protein fingerprints, which have demonstrated to be useful in microorganisms characterization. In this work, protein fingerprints of two species of Staphylococcus grown in different culture media and submitted to temperature and nitrosative stress were studied by CE-LIF. After the growth of the bacteria, protein extracts were obtained by cell lysis using sonication. The water-soluble fraction of these lysates was derivatized on-capillary with a fluorogenic dye, 3-(2-furoyl)quinoline-2-carboxaldehyde. The fluorescent products were analyzed by CE using phosphate buffer containing submicellar concentrations of sodium pentanesulfate and detected by LIF. Different protein fingerprints were obtained depending on the bacterial specie studied, indicating the usefulness of this method for the identification of different species of the same bacterial genus. It was also demonstrated that the CE protein fingerprints were dependent on the culture conditions, such as growth medium, or on stressing conditions, such as heat shock or nitrosative stress. © 2009 Elsevier B.V. All rights reserved.