Protein fingerprinting of Staphylococcus species by capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection

  1. Veledo, M.T. 1
  2. Pelaez-Lorenzo, C. 1
  3. Gonzalez, R. 2
  4. de Frutos, M. 1
  5. Diez-Masa, J.C. 1
  1. 1 Instituto de Química Orgánica General
    info

    Instituto de Química Orgánica General

    Madrid, España

    ROR https://ror.org/05e0q7s59

  2. 2 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

Revista:
Analytica Chimica Acta

ISSN: 0003-2670

Año de publicación: 2010

Volumen: 658

Número: 1

Páginas: 81-86

Tipo: Artículo

DOI: 10.1016/J.ACA.2009.10.053 PMID: 20082778 SCOPUS: 2-s2.0-71749121611 WoS: WOS:000273847500011 GOOGLE SCHOLAR

Otras publicaciones en: Analytica Chimica Acta

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Resumen

Capillary electrophoresis (CE) coupled with laser-induced fluorescence detection (LIF) has allowed to obtain protein fingerprints, which have demonstrated to be useful in microorganisms characterization. In this work, protein fingerprints of two species of Staphylococcus grown in different culture media and submitted to temperature and nitrosative stress were studied by CE-LIF. After the growth of the bacteria, protein extracts were obtained by cell lysis using sonication. The water-soluble fraction of these lysates was derivatized on-capillary with a fluorogenic dye, 3-(2-furoyl)quinoline-2-carboxaldehyde. The fluorescent products were analyzed by CE using phosphate buffer containing submicellar concentrations of sodium pentanesulfate and detected by LIF. Different protein fingerprints were obtained depending on the bacterial specie studied, indicating the usefulness of this method for the identification of different species of the same bacterial genus. It was also demonstrated that the CE protein fingerprints were dependent on the culture conditions, such as growth medium, or on stressing conditions, such as heat shock or nitrosative stress. © 2009 Elsevier B.V. All rights reserved.