Regulación de la citotoxicidad inducida por oleuropeína en células humanas de osteosarcomapapel de la vía apoptótica Bax/Bcl-2

Abstract

Mediterranean diet is widely consumed and accepted in our society. Oleuropein, one of the main components of olive oil, has been investigated in numerous studies, especially those dealing with its anti-cancer properties. The severity of this pathology and the uncontrolled growth it produces in the cells lead to a great biomedical interest in exploring new strategies to efficiently confront this disease. This study evaluated the antiproliferative activity of oleuropein in two human osteosarcoma cell lines (MG-63 and Saos-2). IC50 statistical analysis by Probit regression method showed that oleuropein had similar toxic effects on both cell lines (IC50 ranges from 247.4-475.0 μM for MG-63 cells and from 359.9-798.7 μM for Saos-2 cells). Thereafter, the p-53-null MG-63 human osteosarcoma cells were exposed to oleuropein and the Bax/Bcl-2 apoptotic pathway was investigated. Caspase-3 activity was determined by a colorimetric assay. Furthermore, Bax, Bcl-2 and p-53 mRNA expression was assessed by real-time PCR following 24 hours of exposure. Both the ratio of Bax/Bcl-2 mRNA expression and caspase-3 activity were enhanced in cells exposed to oleuropein when compared to unexposed control cells (P < 0.05). Predictably, no p-53 mRNA expression was observed in MG-63 cells. These results show that oleuropein induces a p-53-independent apoptosis-like mechanism in human MG-63 osteosarcoma cells.