Caracterización del cultivo primario epitelial de conjuntiva humana
- Rivas Jara, Luis
- Blazquez-Martinez, Alejandro
- Muñoz Negrete, Francisco José
- López García, Santiago
- Rebolleda Fernández, Gema
- Domínguez, F.
- Pérez-Esteban Rodríguez, Antonio
ISSN: 0365-6691
Argitalpen urtea: 2014
Alea: 89
Zenbakia: 1
Orrialdeak: 10-16
Mota: Artikulua
Beste argitalpen batzuk: Archivos de la Sociedad Española de Oftalmologia
Laburpena
Objective: To evaluate primary cultures from human conjunctiva supplemented with fetal bovine serum, autologous serum, and platelet-rich autologous serum, over human amniotic membrane and lens anterior capsules. Methods: One-hundred and forty-eight human conjunctiva explants were cultured in CnT50® supplemented with 1, 2.5, 5 and 10% fetal bovine serum, autologous serum and platelet-rich autologous serum. Conjunctival samples were incubated at 37 °C, 5% CO₂ and 95% HR, for 3 weeks. Results: The typical phenotype corresponding to conjunctival epithelial cells was present in all primary cultures. Conjunctival cultures had MUC5AC-positive secretory cells, K19-positive conjunctival cells, and MUC4-positive non-secretory conjunctival cells, but were not corneal phenotype (cytokeratin K3-negative) and fibroblasts (CD90-negative). Conclusions: Conjunctiva epithelial progenitor cells were preserved in all cultures; thus, a cell culture in CnT50® supplemented with 1 to 5% autologous serum over human amniotic membrane can provide better information of epithelial cell differentiation for the conjunctival surface reconstruction.