Modulación mediante tratamiento con atorvastatina y/o amlodipino del perfil de proteínas liberadas por placas ateroscleróticas humanas

  1. Mari Carmen Durán 1
  2. José Luis Martín Ventura 1
  3. Shabaz Mohammed 2
  4. María Eugenia González Barderas 1
  5. Luis Miguel Blanco Colio 1
  6. Sebastián Mas Fontao 1
  7. Verónica Moral 1
  8. Melina Vega de Ceniga 3
  9. Antonio Martín Conejero 3
  10. Javier Serrano 3
  11. José Tuñón Fernández 1
  12. Ole T. Jensen 2
  13. Fernando Vivanco 14
  14. Jesús Egido de los Ríos 1
  1. 1 Fundación Jiménez Díaz

    Fundación Jiménez Díaz

    Madrid, España


  2. 2 University College South Denmark

    University College South Denmark

    Esbjerg, Dinamarca


  3. 3 Hospital Clínico San Carlos de Madrid

    Hospital Clínico San Carlos de Madrid

    Madrid, España


  4. 4 Universidad Complutense de Madrid

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Clínica e investigación en arteriosclerosis

ISSN: 1578-1879

Year of publication: 2006

Volume: 18

Issue: 5

Pages: 167-175

Type: Article

DOI: 10.1016/S0214-9168(06)73684-1 DIALNET GOOGLE SCHOLAR

More publications in: Clínica e investigación en arteriosclerosis


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Introduction Analysis of the complex structure of the atheroma plaque is a classical object of cardiovascular research. We studied proteins secreted by the vascular wall, which could be potential plasma markers. Statins or calcium channel blockers modulate the levels of different circulating proteins in patients with diverse cardiovascular diseases. Methods Two-dimensional electrophoresis and mass spectrometry were applied to identify and characterize proteins differentially secreted by atheroma plaque samples incubated ex vivo versus adjacent fibrous segments considered as controls. We also analyzed the modulatory effect of atorvastatin (10-5 M), amlodipine (10-6 M) and the combination of both drugs. Results From an average of 620 proteins detected per gel, a total of 83 proteins secreted by atheroma plaque samples were identified by mass spectrometry: 34 proteins were increased in atheroma plaque supernatants versus control segments while 31 showed decreased secretion levels in atheroma plaques. Different effects were detected after in vitro drug administration to complicated atheroma plaques, depending on the kind of drug and protein considered, although the majority of the proteins identified reverted to normal levels independently of the treatment administered. Conclusion Proteomic analysis characterized a significant number of novel proteins potentially involved in the formation and instability of complicated atherosclerotic plaques. Modulation of these markers by different drugs may help us to understand new potential mechanisms through which these drugs exert their beneficial effects on atherothrombosis.