Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.

  1. Del Campo, R. 2
  2. Tenorio, C. 1
  3. Rubio, C. 2
  4. Castillo, J. 2
  5. Torres, C. 1
  6. Gómez-Lus, R. 2
  1. 1 Universidad de La Rioja

    Universidad de La Rioja

    Logroño, España


  2. 2 Universidad de Zaragoza

    Universidad de Zaragoza

    Zaragoza, España


International Journal of Antimicrobial Agents

ISSN: 0924-8579

Year of publication: 2000

Volume: 15

Issue: 3

Pages: 221-226

Type: Article

DOI: 10.1016/S0924-8579(00)00169-2 PMID: 10926445 SCOPUS: 2-s2.0-0033837396 WoS: WOS:000088206800009 GOOGLE SCHOLAR

More publications in: International Journal of Antimicrobial Agents

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Aminoglycoside resistance was evaluated in 690 enterococcus strains isolated from different clinical sources originating from patients at the University Clinic Hospital of Zaragoza (Spain). The enterococci obtained from clinically significant samples (blood, urine, or exudates) showed more high- level resistance to gentamicin and streptomycin (65 and 42%, respectively) than those isolated from faecal samples (49 and 23%, respectively). Aminoglycoside-modifying enzymes (AME) from 119 of these high-level gentamicin and streptomycin resistant enterococcus strains were studied. The most frequent AMEs found were APH(3') and AAC(6')-APH(2''). More than one enzyme was detected in 71% of the strains (four different enzymes in 5% of the strains). Three Enterococcus faecalis strains had ANT(4')(4'') enzymatic activity. Different enzymatic expressions of the bifunctional enzyme AAC(6')- APH(2'') were demonstrated in strains in which the complete aac(6')-aph(2'') gene was detected by PCR and hybridization: (i) AAC(6') + APH(2'') activity; (ii) AAC(6') only; (iii) APH(2'') only; and (iv) no activity of AAC(6') or APH(2''). (C) 2000 Elsevier Science B.V. and International Society of Chemotherapy.